![]() (c) 2007 Wiley-Liss, Inc.ħ5 FR 20392 - Investigations Regarding Certifications of Eligibility To Apply for Worker Adjustment Assistance In conclusion, the Ortho-Clinical Vitros ECi Anti-HCV test yields a high rate of false-positive results in the low s/co range in our patient population. A final study comparing all four methods side-by-side showed 63% disagreement by Centaur for Vitros ECi low-positive samples, 75% disagreement by RIBA, and 97% disagreement by polymerase chain reaction (PCR). In comparison with RIBA, 100% (77) samples positive by the Vitros ECi test with s/co values less than 12.0 were negative or indeterminate by RIBA. A separate study of 91 samples with signal-to-cutoff (s/co) values less than 8.0 showed that all but one was negative for HCV ribonucleic acid (RNA). Of 94 specimens positive by Vitros ECi, 19% were observed to be negative by Centaur. To ascertain the true anti-HCV status of samples deemed low-positive by the Ortho-Clinical Vitros ECi test, we tested samples using the ADVIA Centaur HCV screen test (Siemens Medical Solutions Diagnostics), the Chiron recombinant immunoblot assay (RIBA) test (Chiron Corp., Emeryville, CA), and the Roche COBAS Amplicor HCV qualitative test ( Roche Diagnostics, Indianapolis, IN) in a series of studies. Food and Drug Administration (FDA)-approved confirmatory methodologies. Watterson, Jeannette M Stallcup, Paulina Escamilla, David Chernay, Patrick Reyes, Alfred Trevino, Sylvia CĪfter observing a high incidence of low positive hepatitis C virus (HCV) antibody screens by the Ortho-Clinical Vitros ECi test (Orthoclinical Diagnostics, Raritan, NJ), we compared results against those obtained using another chemiluminescent analyzer, as well as two U.S. Recently, Roche Diagnostics launched a real-time PCR platform, the LightCycler480Įvaluation of the Ortho-Clinical Diagnostics Vitros ECi Anti-HCV test: comparison with three other methods. One of the most interesting aspects of real-time PCR based on the detection of fluorophoric labeled oligonucleotides is the possibility of being able to detect conveniently multiple targets in the same PCR reaction. Molenkamp, Richard van der Ham, Alwin Schinkel, Janke Beld, Marcel ![]() ![]() Simultaneous detection of five different DNA targets by real-time Taqman PCR using the Roche LightCycler480: Application in viral molecular diagnostics ![]()
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